About us

Aija Linē , Dr. biol.

Scientific director / Senior researcher
Phone: +371808208
E-mail: aija@biomed.lu.lv

Groups

• A. Linē lab

Projects

• Development of a novel microfluidic device for label-free quantification of prostate cancer-derived extracellular vesicles and analysis of their RNA content (PROCEX)
• Clinical utility of extracellular vesicle-enclosed human and microbial transcriptomes
• Prostate cancer-derived extracellular vesicles as liquid biopsies and therapeutic targets
• Support for the integration of the Latvian Biomedical Research and Study Centre into the European Research Area and Infrastructure objects
• Exploitation of extracellular vesicles for precision diagnostics of prostate cancer
• Precision medicine test for the therapeutic decisions and monitoring of treatment response in breast cancer
• Universal Nano-enhancer for a new multiplexing Surface Plasmon Resonance Imaging analysis of miRNAs in multiple sclerosis (NanoPlasmiRNA)
• Cancer-derived extracellular vesicles: function and clinical applications in prostate cancer
• Development of cancer-specific autoantibody assay for early detection of gastric cancer
• Cancer-derived exosomes – a source of novel biomarkers and therapeutic targets for gastrointestinal cancers
• Development of novel anticancer drugs and immunotherapeutic approaches
• Development of diagnostic and prognostic tests for malignancies based on autoantibody detection
• MicroRNA, mRNA and SNPs as biomarkers for the early diagnosis, prognosis and prediction of therapy outcome of lung cancer
• Development of anticancer drugs targeting cancer stem-like cells
• The Integration of Latvian Biomedical Research in the European Research Area
• Development of serological diagnostics test of malignant tumors
• Elaboration of diagnostic methods to determine the risk factors of malignant tumours, to diagnose early cancer and precancerous stages and optimise cancer therapy
• The role of pharmacogenetics in the therapy of oncologic diseases
• European Network for the identification and validation of Antigens and biomarkers in Cancer and their application in clinical Tumour immunology
• Pre-mRNA splicing defects in cancer: frequency and immunogenicity
• Elaboration of a bioinformatics approach for the identification of cancer-specific alternative splice variants
• Molecular characterisation of gastric and colorectal cancer antigens
• Clinical relevance of serologically identified gastric and colorectal cancer antigens

Publications

• International

• Germinal centers determine the prognostic relevance of tertiary lymphoid structures and are impaired by corticosteroids in lung squamous cell carcinoma Silina, K., Soltermann, A., Attar, F.M., Casanova, R., Uckeley, Z.M., Thut, H., Wandres, M., Isajevs, S., Cheng, P., Curioni-Fontecedro, A., Foukas, P., Levesque, M.P., Moch, H.,, A Linē, Van Den Broek, M , Cancer Research, 78 (5), pp. 1308-1320 , 2018
• Proteome Analysis of Colorectal Cancer Cell Line SW480 Released Extracellular Vesicles Nakurte I, Jekabsons K,, E Zandberga, A Ābols, A Linē, Muceniece R , Key Engineering Materials, Vol. 762, pp 3-7. (IF=0.39) , 2018
• Effect of colorectal cancer-derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages Popěna, I.,, A Ābols, Saulite, L., Pleiko, K.,, E Zandberga, Jěkabsons, K.,, E Endzeliņš, Llorente, A.,, A Linē, Riekstiņa, U. , Cell Communication and Signaling, 16 (1), art. no. 17. PMID: 29690889 , 2018
• Extracellular vesicles derived from hypoxic colorectal cancer cells confer metastatic phenotype to non-metastatic cancer cells E Endzeliņš, A Ābols, A Bušs, E Zandberga, Palviainen, M., Siljander, P.,, A Linē , Anticancer Research, 38 (9), pp. 5139-5147. PMID: 30194161 , 2018
• Miniature diamond-anvil cells for FTIR-microspectroscopy of small quantities of biosamples Grube, M., Shvirksts, K., Krafft, C., Kokorevicha, S.,, E Zandberga, A Ābols, A Linē, Kalnenieks, U , Analyst, 143 (15), pp. 3595-3599. PMID: 29961798 , 2018
• Antigen specificity and clinical significance of IgG and IgA autoantibodies produced in situ by tumor-infiltrating B cells in breast cancer. Garaud S, P Zajakins, Buisseret L, Rulle U, Siliņa K, de Wind A, den Eyden GV, Larsimont D, Willard-Gallo K,, A Linē , Frontiers in Immunology. 2018, 20 Nov. doi: 10.3389/fimmu.2018.02660. , 2018
• A simple and universal enzyme-free approach for the detection of multiple microRNAs using a single nanostructured enhancer of surface plasmon resonance imaging Sguassero, A., Artiga, Á., Morasso, C., Jimenez, R.R., Rapún, R.M., Mancuso, R., Agostini, S., Hernis, A., A Ābols, A Linē, Gualerzi, A., Picciolini, S., Bedoni, M., Rovaris, M., Gramatica, F., de la Fuente, J.M., Vanna, R. , Analytical and Bioanalytical Chemistry, . Article in Press. PMID: 30155701 , 2018
• Colorectal cancer cell line SW480 and SW620 released extravascular vesicles: Focus on hypoxia-induced surface proteome changes Nakurte, I., Jekabsons, K., Rembergs, R.,, E Zandberga, A Ābols, A Linē, Muceniece, R. , Anticancer Research, 38 (11), pp. 6133-6138. PMID: 30396929 , 2018
• Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines Théry, C., Witwer, K.W., Aikawa, E.,, A Linē, Zimmermann, P., Zivkovic, A.M., Zocco, D., Zuba-Surma, E.K , Journal of Extracellular Vesicles, 8 (1), art. no. 1535750 , 2018
• Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes Rodríguez M,, C Bajo Santos, Hessvik NP, Lorenz S, Fromm B, Berge V, Sandvig K, A Linē, Llorente A , Mol Cancer, Oct 5;16(1):156 , 2017
• The prevalence of cancer-associated autoantibodies in patients with gastric cancer and progressive grades of premalignant lesions I Meistere, Werner S, P Zajakins, K Siliņa, U Rulle, A Pismennaja, Šantare D, Kikuste I, Isajevs S, Leja M, Kupčinskas L, Kupčinskas J, Jonaitis L, Wu CY, Brenner H,, A Linē, Z Kalniņa , Cancer Epidemiology Biomarkers Prev., Oct;26(10):1564-1574 , 2017
• Depletion of carbonic anhydrase IX abrogates hypoxia-induced overexpression of stanniocalcin-1 in triple negative breast cancer cells E Zandberga, P Zajakins, A Ābols, D Pūpola, Trapencieris P, A Linē , Cancer Biol Ther, Jun 30:1-10 , 2017
• Detection of circulating miRNAs: comparative analysis of extracellular vesicle-incorporated miRNAs and cell-free miRNAs in whole plasma of prostate cancer patients E Endzeliņš, A Berger, Melne V, C Bajo Santos, K Soboļevska, A Ābols, Rodriguez M, Šantare D, Rudņickiha A, Lietuvietis V, Llorente A, A Linē , BMC Cancer, Nov 9;17(1):730. , 2017
• A novel 3D heterotypic spheroid model for studying extracellular vesicle-mediated tumour and immune cell communication L Sadovska, E Zandberga, Sagini K, Jēkabsons K, Riekstiņa U,, Z Kalniņa, Llorente A, A Linē , Biochem Biophys Res Commun. Dec 14. PubMed PMID: 29248729 , 2017
• Diagnostic, prognostic and predictive value of cell-free miRNAs in prostate cancer: A systematic review E Endzeliņš, Melne, V., Z Kalniņa, Lietuvietis, V., Riekstiņa, U., Llorente, A., A Linē , Molecular Cancer, 15 (1), art. no. 41 , 2016

• Local

• Antigen Reduction Applied to Support Vector Machine Classification for Melanoma-Related Diagnostics. V Jumuts, P Zajakins, A Linē , Polish J. of Environ. Stud 18(4a): 3-8 , 2009
• Evaluation of Breath Test Data and CYP450 Polymorphism for Estimation of Efficiency and Safety of Paclitaxel Chemotherapy. Ozola L, Rostoka E, Vikmanis U, Zvirbule Ž, A Linē, Nakurte I, Plinte I, Mekšs P, Sudraba A, Leja M , Acta Universitatis Latviensis 750: 153 – 9 , 2009
• Applying ANN ensembles to melanomas –related diagnostics on cancer patients. V Jumuts, P Zajakins, A Linē , Scientific Proceedings of Riga Technical University 5: 100-8 , 2008
• Kvercetīna un kemferola ietekme uz iNOS ekspresiju kontroles un LPS inducēta iekaisuma gadījumā Wistar līnijas žurku aknās. Salna E, K Siliņa, Šaripova J, A Linē, Sjakste N , Acta Universitatis Latviensis 735: 114 – 123 , 2008
• One drug does not fit all A Linē, N Līcis , Doctus 3:45-8 , 2005
• Interferon-alpha therapy in chronic myeloid leukemia and detection of bcr/abl transcripts. Bondare D, Žileviča A, Stroža I, Feldmane G, Voltāre A, Kluce G, Petuhovs V, Cīmanis A, Grante H, Teilāne I, Treimane R, A Linē , Proc Latvian Acad Sci, 52 (3/4):102-107. , 1998

• Patents

• Gastric cancer biomarkers and methods of use thereof A Linē, K Siliņa, Z Kalniņa, P Zajakins , European Patent Register EP2620772 , 2012
• Diagnostikas aģents paklitaksela izraisītas aknu toksicitātes noteikšanai. Boka V, Vikmanis U, Zvirbule Ž, Sudraba A, Nakurte I, Leja M, A Linē , LR patents Nr.14097 , 2010

• Conference thesis

• Research results presentation A Linē , European Network on Microvesicles and Exosomes in Heath and Disease (ME-HaD), Roterdama, Nīderlande , 2014
• Characterisation of RNA content in cancer - derived exosomes and microvesicles A Linē, A.Adrejeva, A.Cīrulis, A Ābols, A Zajakina , EACR-23 Biennial Congress of the European association for cancer research, Minhene, Vācija , 2014
• Exploration of the potential of tumour - associated autoantibodies as biomarkers for gastric cancer detection Z Kalniņa, I Meistere, P Zajakins, K Siliņa, A Pismennaja, M. Leja, A Linē , 22nd United European Gastroenterology Week, Vīne, Austrij , 2014
• Cancer autoantibodies as prognostic biomarkers. K Siliņa, P Zajakins, Ancans G, Z Kalniņa, I Meistere, A Pismennaja, Leja M, A Linē , 1st International Symposium on Immunotherapy, The Royal Society in London. Abstract book p22. , 2013
• Autoantibody biomarkers for early detection and prognosis of gastric cancer K Siliņa, P Zajakins, Ancāns G, Z Kalniņa, I Meistere, A Pismennaja, Leja M, A Linē , 15th International Congress of Immunology (ICI), Milan, Italy , 2013
• Cancer-associated autoantibody signatures for early diagnosis and prognosis of gastric cancer K Siliņa, P Zajakins, Ancāns G, Z Kalniņa, I Meistere, A Pismennaja, Leja M, A Linē , B Cell Development and Function Symposium, Keystone, CO, USA. Abstract Book, p121. , 2013
• Identification of autoantibody profiles in melanoma patients associated with the response to immunotherapy. K Siliņa, P Zajakins, Z Kalniņa, I Meistere, A Linē , 12th International Conference on Progress in Vaccination Against Cancer (PIVAC12). Nottingham, UK. Abstract book p30. , 2012
• Role of hypoxia inducible carbonic anhydrase IX in cancer stem cell phenotype. L Ivanova, E Zandberga, A Ābols, K Siliņa, A Linē , EMBL conference Stem cells in cancer and regenerative medicine. Heidelberg. Abstract book, p117. , 2012
• Diagnostic and prognostic relevance of tumour-associated autoantibody signatures in gastric cancer. P Zajakins, Ancāns G, K Siliņa, I Meistere, Z Kalniņa, Leja M, A Linē , EACR 22nd congress. Barcelona, Spain. Eur J Cancer, Sup 5, Vol 48, p.S212 , 2012
• Exploration of autoantibody responses during MCA-induced carcinogenesis. I Meistere, L Sadovska, P Zajakins, A Pismennaja, Z Kalniņa, A Linē , 1st EATI and 3rd ERI-ICP conference on Death, Danger, Inflammation and Immunity. Paris. Abstracts p107. , 2012
• Exosomal miRNA signature as potential biomarker for the early detection of non-small-cell lung cancer. Andrējeva D, E Zandberga, A Ābols, Kopeika U, Purkalne G, P Zajakins, A Linē , International society for extracellular vesicle 2012. Gothenburg, Sweden. Abstract Book, p25. , 2012
• Prognostic and predictive value of autoantibody signatures in melanoma patients. P Zajakins, K Siliņa, Z Kalniņa, I Meistere, A Linē , 1st EATI and 3rd ERI-ICP conference on Death, Danger, Inflammation and Immunity. Paris, France, Abstracts, p117. , 2012
• Increased miR-103/miR-200b ratio contributes to cisplatin resistance in lung cancer by promoting epithelial-to-mesenchymal transition. E Zandberga, A Ābols, Kozirovskis V, Kopeika U, Andrējeva D, Purkalne G, A Linē , EACR conference on Cell Death in Cancer, Amsterdam, Netherlands, Abstracts, P64. , 2012

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