About us

Dace Skrastiņa , Dr. biol.

Leading expert in Laboratory animal science, senior researcher
Phone: +371 25137484
E-mail: daceskr@biomed.lu.lv

Groups

• Dace

Projects

• Immunological characterization of novel gene terapy vectors
• Construction and immunological research of recombinant DNA and RNA vaccine models

Publications

• International

• Comparative protein profiling of B16 mouse melanoma cells susceptible and non-susceptible to alphavirus infection: Effect of the tumor microenvironment J Vasiļevska, De Souza, G.A., Stensland, M., D Skrastiņa, D Žuļenkovs, Paplausks, R., B Ķūrēna, T Kozlovska, A Zajakina , Cancer Biology and Therapy, 17 (10), pp. 1035-1050 , 2016
• Potato Virus M-Like Nanoparticles: Construction and Characterization I Kalnciema, I Baļķe, D Skrastiņa, V Ose-Klinklāva, A Zeltiņš , Molecular Biotechnology, 57 (11-12), pp. 982-992. , 2015
• The Hepatitis B Virus Core Variants that Expose Foreign C-Terminal Insertions on the Outer Surface of Virus-Like Particles A Dišlers, D Skrastiņa, R Renhofa, I Petrovskis, V Ose-Klinklāva, I Liekniņa, J Jansons, P Pumpēns, I Sominska , Molecular Biotechnology, 57 (11-12), pp. 1038-1049 , 2015
• High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model. A Zajakina, J Vasiļevska, D Žuļenkovs, D Skrastiņa, Spaks A, Plotniece A, T Kozlovska , BMC Cancer. 14:460 , 2014
• Silica Nanoparticles as the Adjuvant for the Immunisation of Mice Using Hepatitis B Core Virus-Like Particles. D Skrastiņa, I Petrovskis, I Liekniņa, J Bogans, R Renhofa , PLoS ONE 9(12): e114006 , 2014
• Characterization of human adipose-derived stem cells cultured in autologous serum after subsequent passaging and long term cryopreservation. A Bogdanova-Jātniece, U Bērziņš, Nikulshin S, D Skrastiņa, Ezerta A, D Legzdiņa, T Kozlovska , J Stem Cells. 9(3):135-48 , 2014
• A VLP library of C-terminally truncated Hepatitis B core proteins: correlation of RNA encapsidation with a Th1/Th2 switch in the immune responses of mice. I Sominska, D Skrastiņa, I Petrovskis, A Dišlers, I Bērza, M Mihailova, J Jansons, I Akopjana, I Stahovska, D Dreiliņa, V Ose-Klinklāva, P Pumpēns , PLoS One. Sep 23;8(9):e75938. , 2013
• Chimeric derivatives of hepatitis B virus core particles carrying major epitopes of the rubella virus e1 glycoprotein. D Skrastiņa, I Petrovskis, Petraityte R, I Sominska, V Ose-Klinklāva, I Liekniņa, J Bogans, Sasnauskas K, P Pumpēns , Clin Vaccine Immunol. Nov;20(11):1719-28. , 2013
• Semliki Forest virus biodistribution in tumor-free and 4T1 mammary tumor-bearing mice: a comparison of transgene delivery by recombinant virus particles and naked RNA replicon. J Vasiļevska, D Skrastiņa, K Spunde, Garoff H, T Kozlovska, A Zajakina , Cancer Gene Ther. Jun 22 , 2012
• Recombinant Semliki Forest virus vectors encoding hepatitis B virus small surface and pre-S1 antigens induce broadly reactive neutralizing antibodies B Niedre-Otomere, A Bogdanova-Jātniece, D Skrastiņa, A Zajakina, R Brūvere, V Ose-Klinklāva, Gerlich WH, Garoff H, P Pumpēns, Glebe D, T Kozlovska , Journal of Viral Hepatitis, May 17 , 2012
• Potato Virus Y-Like Particles as a New Carrier for the Presentation of Foreign Protein Stretches I Kalnciema, D Skrastiņa, V Ose-Klinklāva, P Pumpēns, A Zeltiņš , Mol Biotechnol. Dec 14. [Epub ahead of print]. , 2011
• N-terminal myristoylation - dependent masking of neutralizing epitopes in the preS1 attachment site of hepatitis B virus Bremer CM, I Sominska, D Skrastiņa, P Pumpēns, Wahed AA EI, Beutling U, Frank R, Fritz HJ, Hunsmann G, Gerlich WH, Glebe D , J Hepatol. Jul;55(1):29-37. , 2011
• Construction and immunological evaluation of multivalent hepatitis B virus (HBV) core virus-like particles carrying HBV and HCV epitopes. I Sominska, D Skrastiņa, A Dišlers, Vasiljev D, M Mihailova, V Ose-Klinklāva, D Dreiliņa, P Pumpēns , Clin Vaccine Immunol. 17(6):1027-33 , 2010
• Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen. Alekseeva J, I Sominska, D Skrastiņa, Egorova I, Starodubova E, Kushners E, M Mihailova, Petrakova N, R Brūvere, T Kozlovska, Isaguliants M, P Pumpēns , Genet Vaccines Ther. 7:7 , 2009
• High immunogenicity of a hydrophilic component of the hepatitis B virus preS1 sequence exposed on the surface of three virus-like particle carriers. D Skrastiņa, Bulavaite A, I Sominska, Kovalevska L, V Ose-Klinklāva, D Priede, P Pumpēns, Sasnauskas K , Vaccine 26(16):1972-81 , 2008

• Local

• Expression and characterization of the Ryegrass mottle virus non-structural proteins I Baļķe, G Reseviča, D Skrastiņa, A Zeltiņš , Proc. Latvian Acad. Sci., Section B 64(5/6) , 2010
• Display vectors. I. Hepatitis B core particle as a display moiety. Borisova G, Borschukova O, D Skrastiņa, Mezule G, A Dišlers, I Petrovskis, V Ose-Klinklāva, Gusars I, P Pumpēns, E Grēns , Proc. Latvian Acad. Sci. 51:1-7 , 1997

• Patents

• COUPLING OF BORRELIAL PROTEIN BB0689 TO VIRUS-LIKE PARTICLES FOR GENERATION OF LYME DISEASE VACCINE K Tārs, I Petrovskis, A Dišlers, D Skrastiņa, A Zeltiņš, K Brangulis, S Koteloviča , Europena Patent Office, Application number EP15182845.6 , 2015
• Anti-HCV individuālas terapeitiskas vakcīnas prototips I Sominska, J Jansons, P Pumpēns, I Petrovskis, D Skrastiņa, G. Sudmale , LR patents LV 14987 , 2013
• Upeņu reversijas vīrusa antigēnu iegūšana A Zeltiņš, I Baļķe, J Šaripo, I. Moročko-Bičevska, D Skrastiņa , LR patents LV 15018 , 2013
• Heterologas proteīnu sekvences eksponējošu kartupeļu vīrusam PVY līdzīgo daļiņu iegūšana un izmantošana. A Zeltiņš, I Kalnciema, D Skrastiņa, V Ose-Klinklāva, P Pumpēns , LR patents Nr. 14204 , 2010
• Prokariotiska un eikariotiska ekspresijas vektoru komplekts himēra HBV nukleokapsīda ekspresijai un kombinētas anti-HBV un anti-HCV imunoatbildes iegūšanai. I Sominska, M Mihailova, J Jansons, D Skrastiņa, J Aleksejeva, I Petrovskis, P Pumpēns , LR patents Nr. 14161 , 2010

• Conference thesis

• Intratumoral delivery and biodistribution of oncolytic alphaviral vectors in mouse melanoma model. J Vasiļevska, D Skrastiņa, S Lubina, T Kozlovska, A Zajakina , The European Society of Gene and Cell Therapy Congress. Versailles, France. Abstract Book, p. A102. , 2012
• Individualized Anti-HCV/HBV Vaccine Prototype. I Sominska, D Skrastiņa, I Petrovskis, V Ose-Klinklāva, I Akopjana, I Stahovska, P Pumpēns , IX Annual Conference of the New Visby Network on Hepatitis C. Saint Petersburg, Russia. , 2012
• Rekombinanto alfavīrusu vektoru pretvçþa aktivitāte in vivo. A Zajakina, J Vasiļevska, D Skrastiņa, T Kozlovska , Apvienotais Pasaules latviešu zinātnieku III kongress un Letonikas IV kongress „Zinātne, sabiedrība un nacionālā identitāte”. Latvijā, Rīgā , 2011

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