About us

Tatjana Kozlovska , Dr. habil. biol.

Senior researcher
Phone: +371 67808211
E-mail: tatyana@biomed.lu.lv

Groups

• T. Kozlovska lab

Projects

• Development of novel anticancer drugs and immunotherapeutic approaches
• Cell therapy for type 1 diabetes treatment: a novel approach to treat insulin deficiency
• Elaboration of delivery system for DNA, RNA, protein, peptide, and smal molecule medical preparations
• Novel model of HBV infection and a technology for antiviral agent evaluation
• The immunomodulatory properties 1n vitro and safety of human stem cells for type 1 diabetits patients
• Human mesenhymal stem cells diferenciation and optimization into in vitro hepatocytes system
• Modelling of HBV virus replication

Publications

• International

• The True Story and Advantages of RNA Phage Capsids as Nanotools P Pumpēns, R Renhofa, A Dišlers, T Kozlovska, V Ose-Klinklāva, Pushko, P., K Tārs, E Grēns, Bachmann, M.F. , Intervirology, pp. 74-110 , 2016
• Comparative protein profiling of B16 mouse melanoma cells susceptible and non-susceptible to alphavirus infection: Effect of the tumor microenvironment J Vasiļevska, De Souza, G.A., Stensland, M., D Skrastiņa, D Žuļenkovs, Paplausks, R., B Ķūrēna, T Kozlovska, A Zajakina , Cancer Biology and Therapy, 17 (10), pp. 1035-1050 , 2016
• Characterization of senescence of culture-expanded human adipose-derived mesenchymal stem cells D Legzdiņa, Romanauska, A., Nikulshin, S., T Kozlovska, U Bērziņš , International Journal of Stem Cells, 9 (1), pp. 124-136 , 2016
• Studies of the physicochemical and structural properties of self-assembling cationic pyridine derivatives as gene delivery agents Petrichenko, O., Rucins, M., A Vežāne, I Timofējeva, ., Sobolev, A., Cekavicus, B., Pajuste, K., Plotniece, M., Gosteva, M., T Kozlovska, Plotniece, A. , Chemistry and Physics of Lipids, 191, art. no. 4403, pp. 25-37 , 2015
• Alphaviral Vectors for Cancer Treatment A Zajakina, J Vasiļevska, T Kozlovska, Lundstrom K. , Viral Nanotechnology. Jul 2015 , pages 467 -486 , 2015
• Semliki Forest virus expression system as a model for investigating the nuclear import and export of hepatitis B virus nucleocapsid protein. A Zajakina, R Brūvere, T Kozlovska , Acta Virol. 58(2):173-9 , 2014
• High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model. A Zajakina, J Vasiļevska, D Žuļenkovs, D Skrastiņa, Spaks A, Plotniece A, T Kozlovska , BMC Cancer. 14:460 , 2014
• Characterization of human adipose-derived stem cells cultured in autologous serum after subsequent passaging and long term cryopreservation. A Bogdanova-Jātniece, U Bērziņš, Nikulshin S, D Skrastiņa, Ezerta A, D Legzdiņa, T Kozlovska , J Stem Cells. 9(3):135-48 , 2014
• Growth properties and pluripotency marker expression of spontaneously formed thre-dimensional aggregates of human adipose-derived stem cells . A Bogdanova-Jātniece, U Bērziņš, T Kozlovska , Int J Stem Cells. 7(2):143-52 , 2014
• Application of oncolytic alphaviral vectors in combination with 5-fluorouracil for cancer treatment A Zajakina, J Vasiļevska, A. Ezerte, T Kozlovska , Химия структура и функция биомолекул, Minska, Baltkrievija , 2014
• Posttranslational modifications and secretion efficiency of immunogenic hepatitis B virus L protein deletion variants. B Niedre-Otomere, A Bogdanova-Jātniece, R Brūvere, V Ose-Klinklāva, Gerlich WH, P Pumpēns, Glebe D, T Kozlovska , Virol J. Feb 25;10:63. , 2013
• Semliki Forest virus biodistribution in tumor-free and 4T1 mammary tumor-bearing mice: a comparison of transgene delivery by recombinant virus particles and naked RNA replicon. J Vasiļevska, D Skrastiņa, K Spunde, Garoff H, T Kozlovska, A Zajakina , Cancer Gene Ther. Jun 22 , 2012
• Recombinant Semliki Forest virus vectors encoding hepatitis B virus small surface and pre-S1 antigens induce broadly reactive neutralizing antibodies B Niedre-Otomere, A Bogdanova-Jātniece, D Skrastiņa, A Zajakina, R Brūvere, V Ose-Klinklāva, Gerlich WH, Garoff H, P Pumpēns, Glebe D, T Kozlovska , Journal of Viral Hepatitis, May 17 , 2012
• Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen. Alekseeva J, I Sominska, D Skrastiņa, Egorova I, Starodubova E, Kushners E, M Mihailova, Petrakova N, R Brūvere, T Kozlovska, Isaguliants M, P Pumpēns , Genet Vaccines Ther. 7:7 , 2009
• The recurrence of iatrogenic femoral artery pseudoaneurysm after occlusion by ultrasound guided percutaneous thrombin injection. Madaric J, Mistrik A, Vulev I, Liska B, Vozar M, Lederer P, Gocar M,, T Kozlovska, Fridrich V, De Bruyne B, Bartunek J , EuroIntervention 5(4):443-7 , 2009

• Local

• Comparison of ultracentrifugation methods for concentration of recombinant alphaviruses: sucrose and iodixanol cushions V Hutornojs, B Niedre-Otomere, T Kozlovska, A Zajakina , Environmental and Experimental Biology 10: 117–123 , 2012
• Adipose-derived stem cells cultured in autologous serum maintain the characteristics of mesenchymal stem cells. A Bogdanova-Jātniece, U Bērziņš, R Brūvere, T Kozlovska , Proc. Latvian Acad. Sci. 64(3/4): 20–30 , 2010
• Jaunas tendences cilvēka embrionālo cilmes šūnu iegūšanā. U Bērziņš, B Niedre-Otomere, T Kozlovska , Latvijas Ārsts 1: 62-5 , 2008
• Ienaidnieks jāpazīst B Niedre-Otomere, U Bērziņš, A Zajakina, T Kozlovska , Terra 2:18-20 , 2008
• Intracellular localisation of Hepatitis B virus core protein expessed from two different constructs in the Semliki Forest virus expression system Braun S, R Brūvere, A Zajakina, Aleksejeva J, T Kozlovska , Acta Universitatis Latviensis 745: 7-15 , 2008
• Expresion of hepatitis C virus structural genes controlled by alphaviral recombinant replicons Aleksejeva J, I Sominska, R Brūvere, V Ose-Klinklāva, A Zajakina, T Kozlovska , Acta Universitatis Latviensis 745: 115-130 , 2008
• Synthesis of all Hepatitis B structural proteins in Semliki Forest virus expression system. T Kozlovska, A Zajakina, V Ose-Klinklāva, R Brūvere, Alekseeva J, P Pumpēns, Garoff H , Acta Universitatis Latviensis 676: 39-51 , 2004
• New experimental approach for production of HBV virion-like particles in eukaryotic cell lines by SFV-replicon-driven expression. A Zajakina, V Ose-Klinklāva, Garoff H, T Kozlovska , Proc. Latvian Acad. Sci. 58: 49-54 , 2004
• Subcellular localisation of internally deleted HBV core proteins. R Brūvere, Braun S, A Zajakina, Aleksejeva J, Sharipo A, Meisel H, P Pumpēns, T Kozlovska , Acta Universitatis Latviensis 676: 53-64 , 2004
• Specific diagnostics of viral hepatitis B. Current problems. Viksna L, I Sominska, T Kozlovska, J Jansons, P Pumpēns, Arsa F, Rozentale B, Sondore V , LMA Vestis 210-213 , 2000
• Display vectors. II. Recombinant capsid of RNA bacteriophage Qb as a display moiety. T Kozlovska, I Cielēns, I Vasiļjeva, Bundule M, Strelnikova A, A Kazāks, A Dišlers, D Dreiliņa, V Ose-Klinklāva, Gusars I, P Pumpēns , Proc. Latvian Acad. Sci. 51: 8-12 , 1997

• Patents

• Paņēmiens hepatīta B vīrusa infekcijas modeļa izveidošanai A Zajakina, U Bērziņš, T Kozlovska, P Pumpēns, D Legzdiņa, Sondore V., Keišs J., Vīksna L., I Sominska, Arša F. , LR patents LV 14760 , 2013
• Manufacturing method by bioengineering technology for producing human alpha natriuretic peptide. Berzins V, I Jansone, V Baumanis, Skangals A, T Kozlovska, Pusko P, E Grēns , Korean Patent, Nr. 95. 25099 , 1996

• Monographs

• Alphaviruses: multiplicity of vectors and their promising application as vaccines and cancer therapy agents. /in "Medicinal Protein Engineering." pp. 519-532 A Zajakina, B Niedre-Otomere, Alekseeva J, T Kozlovska , CRC Press, Taylor & Francis Group, Boca Raton London, New York , 2009

• Conference thesis

• Hepatitis B virus nucleocapsid particles produced in eukaryotic cells: Properties and purification K Spunde, A.Eihentale, A Zajakina, T Kozlovska, P Pumpēns , European Biotechnology Congress 2014 , 2014
• In search of collaboration T Kozlovska , Harmonizing Integrating vitalizing European research on AIDS and HIV, Budapešta, Ungārija , 2014
• In vitro evaluation of cytotoxic potential of alphaviral vectors in combination with chemotherapeutic agent for cancer treatment. A Zajakina, J Vasiļevska, Spaks A, T Kozlovska , EJC Vol. 48, Suppl. 6, p.59. , 2012
• Intratumoral delivery and biodistribution of oncolytic alphaviral vectors in mouse melanoma model. J Vasiļevska, D Skrastiņa, S Lubina, T Kozlovska, A Zajakina , The European Society of Gene and Cell Therapy Congress. Versailles, France. Abstract Book, p. A102. , 2012
• Synthesis and Studies of New Gene Delivery Agents on Base of Cationic 1,4-Dihydropyridine Derivatives. Kore K, A Vežāne, Rucins M, Cekavicus B, I Timofējeva, Gosteva M, Pajuste K, Jakovele L, Pajuste K, Plotniece M, T Kozlovska, Sobolev A, Plotniece A , International Conference in Pharmacology "Targeting Cellular Regulatory Systems". Rīga, Latvia. Abstract Book, pp. 46-46. , 2012
• Jaunu - liposomas veidojošu katjonu karbazola atvasinājumu izmantošana šūnu transfekcijā in vitro. A Vežāne, G Apsīte, Baran A, R Brūvere, V Ose-Klinklāva, Plotniece A, I Timofējeva, T Kozlovska , Development and Application of New Fluorescent Materials and Methods. Daugavpils, Latvija. bstract Book, p.43. , 2012
• Polyfunctional Gene Transfection Agents. Duburs G, Plotniece A, T Kozlovska, I Timofējeva, A Vežāne, Chekavichus B, Pajuste K, Skrivele B, Vigante B, Kaldre D, Rucins M , 22nd International Symposium on Medicinal Chemistry. Berlin, Germany. P420. , 2012
• Expression of HCV Genome Fragments by Alphavirus Replicon Vektors. Presentation of individual Hepatitis C Hipervariable region. Aleksejeva E, I Sominska, J Jansons, T Kozlovska , IX Annual Conference of the New Visby Network on Hepatitis C. Saint Petersburg, Russia. , 2012
• Трансфекция эукариотических клеток in vitro синтезированной РНK A Vežāne, Duburs G, Plotniece A, I Timofējeva, T Kozlovska , „Биология - наука XXI века” , Krievijā, Puščino , 2011
• Eikariotu šūnu transfekcija ar DNS un IN VITRO sintezētu RNS liposomu kompleksu veidā A Vežāne, Duburs G, Plotniece A, I Timofējeva, T Kozlovska , Apvienotais Pasaules latviešu zinātnieku III kongress un Letonikas IV kongress „Zinātne, sabiedrība un nacionālā identitāte”. Latvijā, Rīgā , 2011
• Rekombinanto alfavīrusu vektoru pretvçþa aktivitāte in vivo. A Zajakina, J Vasiļevska, D Skrastiņa, T Kozlovska , Apvienotais Pasaules latviešu zinātnieku III kongress un Letonikas IV kongress „Zinātne, sabiedrība un nacionālā identitāte”. Latvijā, Rīgā , 2011
• Efficient production of hepatitis B core particles in stably transfected BHK-21 cells K Spunde, A Zajakina, J. Aleksējeva, T Kozlovska , European Biotechnology Congress 2011, Istanbul, Turkey , 2011

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