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Molecular design of new luminescent compounds for diagnostic purposes



Project Title: „Molecular design of new luminescent compounds for diagnostic purposes”

Funding: European Regional Development Fund (ERDF), Measure “Industry-Driven Research”

Project Nr.:

Period: 1st March 2017 – 29 st February 2020

Project costs: 580 000,00 EUR 

BMC Principle Investigator: Dr. biol. D. Pjanova

Project leading partner: Daugavpils Universitāte


In the framework of the project „Molecular design of new luminescent compounds for diagnostic purposes” the interdisciplinary fundamental research will be carried out, which will ensure the synthesis of new luminescent substances for different diagnostic tools. The project is aimed to the synthesis of new luminescent substances for the development of diagnostics methods in medicine and veterinary medicine. Application of newly prepared dyes opens up new opportunities for progress of innovative non-invasive, early, and express diagnostics at the global level. The project is divided in two stages; the first stage includes elaboration of optimal synthetic approaches for new luminophores, its spectral characterization and binding with biological objects (blood cells, animal, and parasite tissues etc.). The second part involves development of protocols. The project implementation will be based on the collaboration of scientific institutions of several established working groups in a complexity of chemical, parasitological, histological, physical, genomics, and clinical genomics methods. Moreover, during the project will be created several work groups, which include important diagnostic fields in medicine, veterinary medicine and biology. The results of the research will be concentrated on the development of new product prototype and protocols of new methods (e.g. histology, karyotyping, parasitology etc.). The synthesis of new sensored luminophores, developed innovative methods, and protocols will be important in Latvia and Europe after the end of the project as well. Through the realisation of this project will be solved significant questions in different fields of diagnostics and research.

Information published: 01.03.2017.

June 1, 2017 - August 31, 2017

During this reporting period, several cell freezing protocols have been tested and freezing has been found to affect the properties of cell membranes.  The search for the best cell refrigeration protocol continues. In addition, differences in spectral parameters between normal cells in culture (fibroblasts) and melanoma cells in culture were confirmed. Pilot experiments have been initiated to see if colour spectral parameters correlate with tumour cell migration e.g. potential to form metastases.

Information published: 31.08.2017.

September 1, 2017 -  November 31, 2017

In order to analyze whether the spectral parameters of sunthesized dyes correlate with tumor cell migration, an in vitro melanoma cell model is under the development. For that, different melanoma cell cultures and various cytokines, as cell migration initiators, have been tested and model optimization continues. Similarly, work on the analysis of spectral parameters changes for sunthesized dyes in plazma samples from cancer patients and controls continues.

Information published: 30.11.2017.


December 1, 2017 - February 28, 2018

The best results in migration tests were obtained using cytokine TNF-alpha and cell starvation (cell growing in serum-free medium). Clear differences in cell migration were obtained between fibroblast and melanoma cells in culture. Comparison of differences between melanoma cell lines continues. The analysis of spectral changes of fluorescent dyes at the single cell level using confocal scanning microscopy are initiated as well.

Information published: 28.02.2018.

Mājas lapas izstrādi finansēja ERAF aktivitātes projekts Nr. 2010/0196/2DP/ "Latvijas biomedicīnas pētījumu integrācija Eiropas zinātnes telpā".