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Anatolijs Sharipo (Read 20262 times)
Anatolijs Sharipo
16.03.2007 at 11:11:00
 
Folding and degradation of proteins
 
Folding and Degradation of Proteins is the group affiliated to LU BMC since October 2000. The vast majority of the fellows are professional scientists with extensive international experience. We study the mechanisms of ubiquitin-proteasome pathway of protein degradation. Another field of our activity is maturation and folding of polypeptides.  
We work in close collaboration with several groups from
  • Karolinska Institute, Stockholm, Sweden
  • Linkopping University, Linkopping, Sweden
  • Australian National University, Canberra, Australia
  • UAB Barcelona, Spain

Projects
  • Xenbiotic modifying protein CYP450 degradation
  • Protein stabilization sequences from viral genes
  • Regulation of keratocytes by Ub-proteasomal pathway
  • Escort secreting protein ERp29
  • Protein ligases
  • Magnetobacteria and magnetosomes

Research Staff
 

 
Dr Anatolijs Sharipo
Dr Ainars Leonchiks
Dr Inara Line
Dr Marina Teveleva
Zhanna Rudevica
Marina Koulikovska
Janis Bogans

Students

Karina Derkach
Viktorija Kurbatska
Dmitrijs Zhulenkovs
Sabine Skipare
 
Publications
Juhna T, Birzniece D, Larsson S, Zulenkovs D, Sharipo A, Azevedo NF, Menard-Szczebara F, Castagnet S, Feliers C, Keevil CW.  Detection of Escherichia coli in biofilms from pipe samples and coupons in drinking water distribution networks. Appl Environ Microbiol. 2007 Aug 24;
 
Braun S, Zajakina A, Aleksejeva J, Sharipo A, Bruvere R, Ose V, Pumpens P, Garoff H, Meisel H, Kozlovska T.  Proteasomal degradation of core protein variants from chronic hepatitis B patients.  
J Med Virol. 2007 Jul 2;79(9):1312-1321  
 
Cebers A, Erglis K, Wen Q, Ose V, Zeltins A, Sharipo A, Janmey P. Dynamics of magnetotactic bacteria in a rotating magnetic field. Biophys J. 2007 May 25; [Epub ahead of print]
 
Koulikovska M, Podskochy A, Fagerholm P.  The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Acta Ophthalmol Scand. 2005 Oct;83(5):543-8.  
PMID: 16187990 [PubMed - indexed for MEDLINE]
 
Pintacuda G, Moshref A, Leonchiks A, Sharipo A, Otting G.  Site-specific labelling with a metal chelator for protein-structure refinement. J Biomol NMR. 2004 Jul;29(3):351-61. PMID: 15213433 [PubMed - indexed for MEDLINE]
 
Podskochy A, Koulikovska M, Fagerholm P, van der Ploeg I.  Biglycan gene expression in UVR-exposed rabbit corneas. Acta Ophthalmol Scand. 2004 Apr;82(2):200-4. PMID: 15043541 [PubMed - indexed for MEDLINE]
 
Liepinsh E, Barbals R, Dahl E, Sharipo A, Staub E, Otting G.  The death-domain fold of the ASC PYRIN domain, presenting a basis for PYRIN/PYRIN recognition. J Mol Biol. 2003 Oct 3;332(5):1155-63. PMID: 14499617 [PubMed - indexed for MEDLINE]
 
Liepinsh E, Leonchiks A, Sharipo A, Guignard L, Otting G.  Solution structure of the R3H domain from human Smubp-2. J Mol Biol. 2003 Feb 7;326(1):217-23.  
 
Leonchiks A, Stavropoulou V, Sharipo A, Masucci MG.  Inhibition of ubiquitin-dependent proteolysis by a synthetic glycine-alanine repeat peptide that mimics an inhibitory viral sequence. FEBS Lett. 2002 Jul 3;522(1-3):93-8.  
 
Sargsyan E, Baryshev M, Szekely L, Sharipo A, Mkrtchian S.  Identification of ERp29, an doplasmic reticulum lumenal protein, as a new member of the thyroglobulin folding complex. J Biol Chem. 2002 May 10;277(19):17009-15. Epub 2002 Mar 7.  
 
Williams NK, Prosselkov P, Liepinsh E, Line I, Sharipo A, Littler DR, Curmi PM, Otting G, Dixon NE.  In vivo protein cyclization promoted by a circularly permuted Synechocystis sp. PCC6803 DnaB mini-intein. J Biol Chem. 2002 Mar 8;277(10):7790-8. Epub 2001 Dec 12.  
 
Sargsyan E, Baryshev M, Backlund M, Sharipo A, Mkrtchian S.  Genomic organization and promoter characterization of the gene encoding a putative endoplasmic reticulum chaperone, ERp29.
Gene. 2002 Feb 20;285(1-2):127-39.  
 
Heessen S, Leonchiks A, Issaeva N, Sharipo A, Selivanova G, Masucci MG,
Dantuma NP.  Functional p53 chimeras containing the Epstein-Barr virus Gly-Ala repeat are
protected from Mdm2- and HPV-E6-induced proteolysis.
Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1532-7. Epub 2002 Jan 22.  
 
Li L, Sharipo A, Chaves-Olarte E, Masucci MG, Levitsky V, Thelestam M,
Frisan T.  The Haemophilus ducreyi cytolethal distending toxin activates sensors of DNA
damage and repair complexes in proliferating and non-proliferating cells.
Cell Microbiol. 2002 Feb;4(2):87-99.  
 
Dantuma NP, Sharipo A, Masucci MG.  Avoiding proteasomal processing: the case of EBNA1.
Curr Top Microbiol Immunol. 2002;269:23-36. Review.  
 
Koulikovska M, van der Ploeg I, Herrmann B, Montan PG.  Respiratory syncytial virus and chlamydia are not detectable by PCR in ongoing vernal keratoconjunctivitis.
Ocul Immunol Inflamm. 2001 Dec;9(4):253-7.  
 
Sharipo A, Imreh M, Leonchiks A, Branden C, Masucci MG.  
 cis-Inhibition of proteasomal degradation by viral repeats: impact of length
and amino acid composition.
FEBS Lett. 2001 Jun 15;499(1-2):137-42.  
 
Liepinsh E, Baryshev M, Sharipo A, Ingelman-Sundberg M, Otting G, Mkrtchian
S.  Thioredoxin fold as homodimerization module in the putative chaperone ERp29:
NMR structures of the domains and experimental model of the 51 kDa dimer.
Structure. 2001 Jun;9(6):457-71.  
 
Murovska M, Lejniece S, Kozireva S, Koulikovska M, Yin H, Blomberg J.  
 Human retrovirus 5 sequences in peripheral blood cells of patients with B-cell
non-Hodgkin's lymphoma.
Int J Cancer. 2000 Mar 15;85(6):762-70.  
 
Sylvin H, Matvienko O, Leonchiks A, Alving K, van der Ploeg I.  
 Molecular cloning, expression, and purification of pig interleukin-5.
Immunogenetics. 2000 Jan;51(1):59-64.  
 
Leonchiks A, Liepinsh E, Barishev M, Sharipo A, Masucci MG, Otting G.  
 Random coil conformation of a Gly/Ala-rich insert in IkappaB alpha excludes
structural stabilization as the mechanism for protection against proteasomal
degradation.
FEBS Lett. 1998 Dec 4;440(3):365-9.  
 
Sharipo A, Imreh M, Leonchiks A, Imreh S, Masucci MG.  
 A minimal glycine-alanine repeat prevents the interaction of ubiquitinated I
kappaB alpha with the proteasome: a new mechanism for selective inhibition of
proteolysis.
Nat Med. 1998 Aug;4(8):939-44.  
 
Mkrtchian S, Baryshev M, Matvijenko O, Sharipo A, Sandalova T, Schneider G,
Ingelman-Sundberg M.  
 Oligomerization properties of ERp29, an endoplasmic reticulum stress protein.
FEBS Lett. 1998 Jul 24;431(3):322-6. Erratum in: FEBS Lett 1998 Aug
21;433(3):335.
 
Grants
 
LZP grants
A.Sharipo  
2008  
 
Valsts Zinatnes programa/State Research Program
Projekt 11 Jauni pretvēža preparātu skrīninga instrumenti/New anticancer screening systems
Projekt 5 Bioloģiski aktīvo vielu strukturpētījumi un receptora-liganda mijiedarbības modelēšana/Structural Research of Bioactive Molecules  
 
 
ERAF grants
A.Leonchiks  
2006-2008 ERAF grants VPD1/ERAF/CFLA/05/APK/2.5.1./000032/017  Ceļš uz mākslīgu radzeni  
Reports:  
 
Alumni
Aivars Vilnis
Ilze Druka
Ginta Pece
Anzhela Laizane
Julija Peregoudova  
Anita Vitina
Olga Matvijenko
Raitis Barbals
Mikhail Baryshev
Rita Granta
Martins Boroduskis
Andris Grunskis
 
« Last Edit: 08.03.2008 at 17:14:57 by sharip »  
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