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23.04.2010 at 12:56:22
Id number
Identifikācijas nr.
291
date
Izsludināts mājas lapā
23.04.2010 at 12:56:22
Type of purchase
Iepirkuma veids
Purchase/Piegāde
description
Iepirkuma priekšmets
Reaktīvu un aksesuāru piegāde reālā laika PCR (sk. specifikāciju)
price
Paredzamā līgumcena, bez PVN
līdz 19 999,99 Ls gada laikā
price
Līgumcena, bez PVN
līdz 19 999,99 Ls gada laikā LVL
Contractor
Līguma izpildītajs
Adrona SIA; Inoma Baltic Ltd
date
Lēmuma pieņemšanas datums
20.05.2010
Contacts
Kontaktpersona
Svetlana Kazmerika, svetlana@biomed.lu.lv
additional info
Specifikācija
Reaktīvu un aksesuāru piegāde reālā laika PCR

Specifikācija:


1 ABsolute Blue QPCR SYBR Green Low ROX Mix AB-4322/B
* Easy-to-see mix. Master mix contains an inert blue dye for easy sample visualization during reaction set-up.
* Maximum specificity. Chemically-modified Thermo-Start Taq remains completely inactive until heated to 95ºC for 15 minutes, thereby preventing non-specific amplification.
* Improved efficiency. The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time.
* Increased sensitivity. The combination of ThermoStart Taq and dTTP increases sensitivity and allows for detection of low copy number templates.
* Instrument specific. Individually optimized QPCR mixes designed for all major QPCR instruments. Mixes are also available for use with probes or SYBR Green chemistry.
* Reproducible results. All mixes undergo extensive quality control testing to ensure batch-to-batch consistency.
* Simple protocol. Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination.
2 Ultra Clear Caps w/White Tubes
(250 strips) AB-1502/W
Attached cap for individual tube access
Ultra Clear Caps with white tubes for QPCR
Compatible with 0.2ml thermal cycler blocks
Uniform, thin wall design for optimal heat transfer
Manufactured from virgin polypropylene and certified RNase, DNase and endotoxin free
3 96-well QPCR plates White (Non-skirted) AB-0600/W * Specially selected white shade delivers maximum signal reflection for highly sensitive detection of even low copy number templates.
* Opaque well walls prevent signal from being absorbed by the cycler block, further improving sensitivity.
* Well-to-well cross talk is reduced, keeping sample signals isolated, and delivering more reproducibility between technical replicates.
*Adaptable to MicroAmp® Optical Adhesive Film (4311971; Applied Biosystems)
*Used with: QPCR cycler ABI PRISM® 7500, 7900HT Fast Real-Time PCR System with 384-Well Block Module, 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory, 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module, ABI PRISM® 7000 Sequence Detection System (Notebook), AmpErase® Uracil N-glycosylase (UNG), High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, 200 Reactions, High Capacity cDNA Reverse Transcription Kit, 200 Reactions, MicroAmp® Splash Free 96-Well Base, SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL), TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG, TaqMan® Universal PCR Master Mix, 1-Pack (1 x 5 mL), TaqMan® Universal PCR Master Mix, 10-Pack (10 x 5 mL), TaqMan® Universal PCR Master Mix, 5 pack (5 x 5 mL), TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 1 Bulk Pack (1 x 50 mL)
4 ABsolute QPCR Seals (Pressure-sensitive adhesive seal) AB-1170 * Seals have full optical clarity for maximum and consistent signal transmission.
* Proprietary adhesive technology forms an ultra-strong bond to safeguard against evaporation.
* Adhesive is only released on contact with raised well rims, maintaining optical clarity of seal above wells and preventing unwanted exposure to samples.
* Pressure sensitive design allows for easy positioning during application; the seal is freely maneuverable until pressure is applied.
5 Adhesive PCR Foil AB-0626
Applications: Thermal cycling with a screw-down or clip-down lid; sample storage.
Characteristics: Strong adhesive; pierceable with a pipette tip; peelable.
Temperature range: -70ºC to +150ºC
6 1. 96-well 0.8ml Storage Plate (natural colour) AB-0765 Features * 96 round wells with 800µl maximum well volume. * Conical well bottoms for complete sample retrieval. * Raised rim design around each well for optimal sealing and reduced risk of cross contamination. * Suitable for use with DMSO. * Alphanumeric grid-referencing. * Designed according to SBS footprint standards for robotic compatibility. * Wide working temperature range from -196°C to +121°C. * Can be barcoded for improved sample tracking. * Manufactured from virgin medical grade polypropylene and certified RNase, DNase and endotoxin free.Sealing Options * Adhesive Sealing (0.8ml working volume). * Heat sealing (0.8ml working volume). * Storage Plate Cap Strips (0.75ml working volume). * 96 Cap Sealing Mats (0.65ml working volume).Technical Information * Autoclavable. * Max RCF (g) - 200g (0.65ml/well).

7 96-well 2.2ml Storage Plate (natural colour) AB-0661 Features
* 96 square wells with 2.2ml maximum well volume.
* U-bottomed wells for easy sample re-suspension.
* Square well format to maximize storage capacity.
* Raised rim design around each well for optimal sealing and reduced risk of cross contamination.
* Suitable for use with DMSO.
* Alphanumeric grid-referencing.
* Designed according to SBS footprint standards for robotic compatibility.
* Wide working temperature range from -196°C to +121°C.
* Can be barcoded for improved sample tracking.
* Manufactured from virgin medical grade polypropylene and certified RNase, DNase and endotoxin free.
Sealing Options
* Adhesive Sealing (2.2ml working volume)
* Heat sealing (2.2ml working volume)
* 96 Cap Sealing Mats (1.8ml working volume)
Technical Information
* Autoclavable
8 384-Well Microarray Plate, cylindrical wells, natural colour AB-1055 • 384 round wells
• 70µl well volume
• Raised well rims for effective sealing and reduced cross-contamination risk
• Precision moulded with flat profile and strengthening ribs
• Alphanumeric grid-referencing and cut-away corner
• Fully skirted for compatibility with automated systems
• Within SBS footprint recommendations
9 ABsolute™ QPCR Low ROX Mix
AB-1319/b Features:
ABsolute™ QPCR Low ROX Mix has been developed to quantify DNA and cDNA. With the exception of primers and template, this 2X mix contains all the components required to perform a rapid, sensitive and reproducible QPCR reaction:

* Thermo-StartTM DNA Polymerase, a chemically modified hot-start version of Thermoprime Plus DNA Polymerase, which prevents non-specific amplification during the reaction set-up. This enzyme requires an activationstep at 95°C for 15 minutes.
*Proprietary reaction buffer which provides highly sensitive, specific and consistent fluorescence readings for real-time and end-point analysis. This buffer has been optimized for MgCl2 and enhancers to improve amplification across a wide range of templates including plant DNA and GC rich fragments.
*dNTP’s, including dTTP to improve reaction sensitivity and efficiency compared to dUTP.
*ROX, passive reference dye for normalization of data.
*compatible for use with any probe system and QPCR cyclers requiring low ROX dye levels, including ABI PRISM® 7500 (including Fast-Block).
*Pack-size: B-10x5ml
10 Ultra Clear Cap Strips
AB-0866 Features:
* 8-cap strip
*Specially formulated ultra clear polypropylene
*Highly polished
*Flat cap design
*Compatible with all ABgene PCR tube strips and 96-well PCR plates
*Application – Real-time, Quantitative PCR (QPCR), fluorescence detection
*compatible for use with any probe system and QPCR cyclers requiring low ROX dye levels, including ABI PRISM® 7500
11 MicroAmp® Optical Adhesive Film
4311971
*Optical adhesive film,
*Application – Real-time, Quantitative PCR (QPCR),
*DNA/RNA/RNase free,
*Used with: QPCR cycler ABI PRISM® 7500, 7900HT Fast Real-Time PCR System with 384-Well Block Module, 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory, 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module, ABI PRISM® 7000 Sequence Detection System (Notebook), AmpErase® Uracil N-glycosylase (UNG), High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, 200 Reactions, High Capacity cDNA Reverse Transcription Kit, 200 Reactions, MicroAmp™ Optical 384-Well Reaction Plate, MicroAmp™ Optical Film Compression Pad, MicroAmp® Adhesive Film Applicator, MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1 ml, MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1 ml, MicroAmp® Optical 384-Well Reaction Plate with Barcode, MicroAmp® Optical 384-Well Reaction Plate with Barcode, MicroAmp® Optical 384-Well Reaction Plate with Barcode, MicroAmp® Optical 96-Well Reaction Plate, MicroAmp® Optical 96-Well Reaction Plate, MicroAmp® Optical 96-Well Reaction Plate with Barcode, MicroAmp® Optical 96-Well Reaction Plate with Barcode, MicroAmp® Splash Free 96-Well Base, SYBR® Green PCR Master Mix, 1-Pack (1 x 5 mL), TaqMan® Fast Universal PCR Master Mix (2x), No AmpErase® UNG, TaqMan® Universal PCR Master Mix, 1-Pack (1 x 5 mL), TaqMan® Universal PCR Master Mix, 10-Pack (10 x 5 mL), TaqMan® Universal PCR Master Mix, 5 pack (5 x 5 mL), TaqMan® Universal PCR Master Mix, No AmpErase® UNG, 1 Bulk Pack (1 x 50 mL), 96-well QPCR plates White (Non-skirted, AB-0600w).
 
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