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09.07.2010 at 13:03:00
Id number
Identifikācijas nr.
292
date
Izsludināts mājas lapā
09.07.2010 at 13:03:00
Type of purchase
Iepirkuma veids
Purchase/Piegāde
description
Iepirkuma priekšmets
Reaktīvu komplektu miRNS profilēšanai, kvantificēšanai un programmatūras miRNS profila datu analīzei piegāde 24 mēnešu laikā (sk. specifikāciju)
price
Paredzamā līgumcena, bez PVN
līdz 19 999,99 Ls
price
Līgumcena, bez PVN
līdz 28457.42 EUR
Contractor
Līguma izpildītajs
Exiqon A/S
date
Lēmuma pieņemšanas datums
01.09.2010
Contacts
Kontaktpersona
Svetlana Kazmerika, svetlana@biomed.lu.lv
additional info
Specifikācija
Reaktīvu komplektu miRNS profilēšanai, kvantificēšanai un programmatūras miRNS profila datu analīzei piegāde 24 mēnešu laikā

Produktu saraksts (Exiqon kat. Nr. vai ekvivalents produkts)


Npk. Producer Cat. Number Product name Specification Rxns
LNA based microRNA arrays with labeling kit equivalent to:
1 Exiqon 208302-A
miRCURY LNA™ microRNA Array, 5th generation – hsa, mmu & rno, 24 slides
Contains more than 1891 capture probes, covering all human, mouse and rat microRNAs annotated in miRBase 14.0, as well as all viral microRNAs related to these species. In addition, this array contains capture probes for 385 new miRPlus™ human microRNAs. These are proprietary microRNAs not found in miRBase. Based on LNA technology. Ship complete with all array hybridization and washing solutions. 52 synthetic spike-in microRNAs are included in product. These are ideal for use in control experiments, normalizations and more. Sample input of 30 ng total RNA is sufficient and high specificity with 1 nt discrimination.
24 slides
2 Exiqon 208302-A
miRCURY LNA™ microRNA Array, 5th generation – hsa, mmu & rno, 6 slides Contains more than 1891 capture probes, covering all human, mouse and rat microRNAs annotated in miRBase 14.0, as well as all viral microRNAs related to these species. In addition, this array contains capture probes for 385 new miRPlus™ human microRNAs. These are proprietary microRNAs not found in miRBase. Based on LNA technology. Ship complete with all array hybridization and washing solutions. 52 synthetic spike-in microRNAs are included in product. These are ideal for use in control experiments, normalizations and more. Sample input of 30 ng total RNA is sufficient and high specificity with 1 nt discrimination.
6 slides
3 Exiqon 208032-A
miRCURY LNA™ microRNA Hy3/Hy5 Power labeling kit * Simple two-step protocol requiring no small RNA enrichment
* MicroRNA profiles from as low as 30 ng total RNA
* Consistent reliable results
* Uniform labeling
* Compatible with all common microarray scanning equipment. First, 5’-phosphates are removed from the microRNA termini using Calf Intestinal Alkaline Phosphatase (CIP). Second, a fluorescent label is attached enzymatically to the 3’-end of the microRNAs in the total RNA sample. This is followed by an enzyme inactivation step after which the sample is ready for hybridization. Compatible with LNA based microRNA Arrays. 24 rxns
Softwares for data analysis of LNA based microRNA arrays equivalent to:
4 Exiqon 208221
ImaGene®/Nexus™ - 30 day license for 24 slides
* Specially addapted for LNA based microRNA Arrays
* Advanced spot finding and grid placement
* Automatic analysis and quality check of all spots
* Interactive spot checking
* Intuitive interface and navigation
* Scanner, array and system independent
* Integrated settings file for convenient analysis of LNA™ microRNA Arrays
* A quality control template specifically designed for LNA™ microRNA Arrays.
* Simple but powerful workflow – No bioinformatics/programming skills required
* One click workflow, with direct links to microRNAs in miRBase and TargetScan
* Combines replicate measurements
* Identifies differentially expressed microRNAs
* Clusters analysis: clusters sample groups or microRNAs 1
5 Exiqon 208220
ImaGene®/Nexus™ - Perpetual license * Specially addapted for LNA based microRNA Arrays
* Advanced spot finding and grid placement
* Automatic analysis and quality check of all spots
* Interactive spot checking
* Intuitive interface and navigation
* Scanner, array and system independent
* Integrated settings file for convenient analysis of LNA™ microRNA Arrays
* A quality control template specifically designed for LNA™ microRNA Arrays.
* Simple but powerful workflow – No bioinformatics/programming skills required
* One click workflow, with direct links to microRNAs in miRBase and TargetScan
* Combines replicate measurements
* Identifies differentially expressed microRNAs
* Clusters analysis: clusters sample groups or microRNAs 1
microRNA amplification kit set based on LNA technology equivalent to:
6 Exiqon 203300
Universal cDNA Synthesis Kit, 16-32 rxns
The kit contains eaģents for fast and reliable reverse transcription of all microRNAs into cDNA in a single reaction step. The number of pipetting steps are kept to a eaģents order to save time and eaģents technical variation. Fast, easy and reliable first strand synthesis, high performance SYBR® Green PCR master mix, includes RNA spike-in template and Control Primer set for monitoring performance. The RNA spike-in can be used with the Control primer set provided in the SYBR® Green master mix, Universal RT eaģe the microRNA Ready-to-use PCR panels as a positive control and/or as an inter-plate calibrator. 16-32 rxns
7 Exiqon 203400 SYBR Green master mix, Universal RT, 25ml Kit contain the appropriate volume of 2x SYBR® Green master mix, nuclease-free water and a Control primer set. The Control primer eaģen designed for amplification of the synthetic RNA spike-in provided in the Universal cDNA synthesis kit. The detection is based on SYBR® Green which allows quality control of the resulting PCR amplicon by melt curve analysis. The LNA™-enhanced primer sets require eaģents PCR reaction conditions for optimal performance. The SYBR® Green master mix have been developed and validated using the LNA™-enhanced primer sets, use of eaģents for real-time PCR, that are not validated for LNA enhanced primers will affect the quality of the results 2500 (20 l) -5000 (10 l) rxns
8 Exiqon 203450
SYBR Green master mix, Universal RT, 2,5ml
Kit contain the appropriate volume of 2x SYBR® Green master mix, nuclease-free water and a Control primer set. The Control primer eaģen designed for amplification of the synthetic RNA spike-in provided in the Universal cDNA synthesis kit. The detection is based on SYBR® Green which allows quality control of the resulting PCR amplicon by melt curve analysis. The LNA™-enhanced primer sets require eaģents PCR reaction conditions for optimal performance. The SYBR® Green master mix have been developed and validated using the LNA™-enhanced primer sets, use of eaģents for real-time PCR, that are not validated for LNA enhanced primers will affect the quality of the results 250 (20 l) -500 (10 l) rxns
9 Exiqon 204000-206999 hsa-miR-xxx, LNA™ PCR primer set * Unmatched sensitivity- start microRNA quantification from just 1pg total RNA
* Ideal for samples with low RNA yield such as serum/plasma, FFPE sections and LCM samples
* High specificity – discrimination of closely related microRNAs and between mature and precursor microRNAs
* Fast and easy-to-use – simple two-step protocol takes less than 3 hours
* Reliable performance- fully validated and optimized LNA™ enhanced primers. 100 rxns
10 Exiqon 203901-203909 Endogenous control PCR primer set, UniRT * Accurate and reliable normalization of microRNA real-time PCR data
* Optimized primer sets for the quantification of endogenous small nucleolar or nuclear RNA molecules
* These primer sets target endogenous small non-coding RNAs that are constitutively expressed in a wide variety of tissues. 100 rxns


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